Siv
Lub zog^®Adenovirus nrawm sim ntaus ntawv (cov quav) yog qhov pom kev saiimmunoassay rau cov kev ua tau zoo nrhiav kom tau txais ntawm adenovirus hauv tib neegquav qaub. Cov khoom siv no yog npaj rau kev siv raws li kev pab cuam hauv kev kuaj mob ntawm adenovirus
muaj kab mob.

Lub hauv paus rau phau ntawv
Ingic Adenoviruses, feem ntau AD40 thiab AD41, yog ib qho ua rau muaj mob zawv plabHauv ntau tus menyuam yaus uas raug kev txom nyem los ntawm cov kab mob diarreal, thib obtsuas yog rau lub rauv. Tus kab mob diarrheal yog qhov tseem ceeb ua rau tuagHauv cov menyuam yaus thoob ntiaj teb, tshwj xeeb hauv cov tebchaws. Adenoviruspathogens tau sib cais thoob plaws lub ntiaj teb, thiab tuaj yeem ua rau zawv plabHauv hoob menyuam puag ncig. Cov kab mob feem ntau pom hauv cov menyuam yaus tsawg duaOb xyoos lub hnub nyoog, tab sis tau pom hauv cov neeg mob txhua lub hnub nyoog.Cov kev tshawb fawb qhia tau tias adenoviruses cuam tshuam nrog 4-15% ntawm txhua qhovCov neeg nyob hauv tsev kho mob ntawm kis tus kab mob.

Kev kuaj mob sai thiab raug mob ntawm adenovirus-hais txog cov pa roj hmab muaj feem xyuamNyob rau hauv kev tsim cov etiology ntawm Gastroentitis thiab hais txog tus neeg tswj hwm tus neeg mob.Lwm yam kev kuaj mob hauv tshuab hluav taws xob (em) thiabNucleic acid hybridization yog kim thiab ua haujlwm-ua kom muaj zog. Muab covtus kheej kev txwv ntawm tus kheej ntawm adenovirus kis, zoo li kim thiabYuav tsis tsim nyog cov zog ua haujlwm tsis tsim nyog.

Lub ntsiab lus
Lub Adenovirus Sai Sai Ntaus Ntaus (Cov Khaub Ncaws) Pom Adenoviruslos ntawm kev pom kev sib xws ntawm cov xim tsim kho ntawm sab hauvSawb. Anti-Adenovirus tshuaj tiv thaiv kab mob yog immobilized rau ntawm thaj tsam xeem ntawmmembrane. Thaum kuaj, qhov hnoos qeev reacts nrog anti-adenovirus tshuaj tiv thaivconjoulated rau cov xim sau thiab prepoated rau cov qauv ncoo ntawm qhov kev xeem.Cov sib tov ces mig los ntawm daim nyias nyias los ntawm capillary kev ua thiab cuam tshuamnrog reagents ntawm daim nyias nyias. Yog hais tias muaj txaus adenovirus hauv qhov quav, aCov kob band yuav tsim nyob rau ntawm thaj chaw kuaj ntawm daim nyias nyias. Lub xub ntiag ntawm nokob band qhia tias muaj txiaj ntsig zoo, thaum nws tsis yog qhov tsis zootshwm sim. Cov tsos ntawm cov kob ntawm cov cheeb tsam tswj tau ua haujlwmtxheej txheem tswj, qhia tias cov ntim ntim kom zoo tauntxiv thiab membrane wicking tau tshwm sim.

Kev ua
Nqa cov ntawv xeem, cov quav, ntas thiab / lossis tswj rau chav kub(15-30 ° C) ua ntej siv.
1. Cov ntawv sau cia thiab kev kho mob ua ntej:
1) Siv cov thawv ntim huv, kom qhuav rau cov sau cia. Cov txiaj ntsig zoo tshaj plaws yuav yogTau txais yog hais tias cov assay yog ua nyob rau hauv 6 teev tom qab sau.
2) Rau cov khoom siv khoom khov: unscrew thiab tshem tawm cov neeg thov raj. YuavCeev faj kom tsis txhob nchuav los yog kev daws teeb meem los ntawm lub raj. Sau cov hnoos qeevlos ntawm kev tso cov ntawv thov mus rau hauv tsawg kawg 3 qhov chaw sib txawv ntawm covCov quav kom tau mus sau kwv yees li 50 mg ntawm cov quav (sib npaug rau 1/4 ntawm pea).Rau cov hnoos qeev: tuav lub pipette vertically, aspirate quavcov quav, thiab tom qab ntawd hloov 2 dops (kwv yees li 80 μl) rau hauvhnoos qeev sau cov raj uas muaj cov extraction tsis.
3) Hloov tus neeg thov rov qab mus rau hauv lub raj thiab ntsia hlau lub hau kom nruj. YuavCeev faj kom tsis txhob tawg qhov ntxeev ntawm cov dej ua kom huv.
4) co qhov hnoos qeev lub raj siv zog sib xyaw kom sib xyaw cov hnoos qeev thiabcov extraction tsis. Cov ntawv qhia npaj npaj nyob rau hauv lub towimen sau rajtej zaum yuav khaws cia rau 6 lub hlis ntawm -20 ° C yog tias tsis kuaj nyob rau hauv 1 teev tom qabkev npaj.

2. Kuaj
1) Tshem tawm cov kev xeem los ntawm nws lub hnab ntim khoom, thiab muab nws tso rauib qib huv thiab theem. Sau npe rau qhov ntsuas nrog tus neeg mob lossis tswjcim npe. Rau cov txiaj ntsig zoo tshaj plaws, qhov assay yuav tsum ua nyob rau hauv ibteev.
2) Siv ib daim ntawv ntawm daim ntaub, tawg cov lus ntxeev ntawm cov raj ua haujlwm. Tuavlub raj kab ntsug thiab dispense 3 tee ntawm kev daws rau hauv cov hnoos qeev(S) ntawm cov cuab yeej xeem.Zam kev ua txhaum cua npuas hauv cov hnoos qeev, thiab tsis ntxiv
Ib qho kev daws teeb meem rau lub qhov rais.Raws li qhov kev sim pib ua haujlwm, xim yuav migrate hla daim nyias nyias.

3. Tos kom cov xim band (s) tshwm sim. Qhov tshwm sim yuav tsum tau nyeem ntawm 10feeb. Tsis txhais qhov tshwm sim tom qab 20 feeb.

Nco tseg:Yog tias qhov quav me me tsis migrate vim muaj cov khoom siv, centrifugeCov qauv sau cia muaj nyob hauv cov tsis sib thooj. Sau 100 μl ntawmSupernatant, faib rau hauv cov hnoos qeev zoo (cov) ntawm cov cuab yeej sim tshiab thiab pib dua, ua raws cov lus qhia saum toj no.

Ntawv muaj