Siv
Lub zog^®Cryptococccal antigen kev sim sai saiantigens ntawm cryptococcus hom complex (cryptococcus neoformans thiabCryptococcus gattii) hauv ntshav dej hiav txwv, ntshav, tag nrho cov ntshav thiab cov ntses hlwb(CSF). Lub assay yog cov tshuaj uas siv tshuaj nqa-kev kuaj cov khoom lag luam uas tuaj yeem pab tau nyob rau hauvKev kuaj mob ntawm cryptococosis.

Lub hauv paus rau phau ntawv
Cryptococcos yog tshwm sim los ntawm ob hom tsiaj ntawm lub cryptococcus hom complex(Cryptococcus neoformans thiab cryptococcus gattii). Cov tib neeg nrog tsis muaj zogKev tiv thaiv xovtooj ntawm tes yog qhov muaj feem ntau txaus ntshai ntawm kev kis mob. Cryptococcosis yog ib qhontawm feem ntau cov kev coj noj coj ua ntawm cov neeg mob AIDS. Nrhiav tau ntawmcryptococcal antigen hauv ntshav dej thiab CSF tau nthuav dav ntau yamKev siab siab thiab qhov tshwj xeeb.

Lub ntsiab lus
Lub zog^®Cryptococcal antigen kev sim sai sai xeem tau tsim losTxheeb Cryptococcus hom kev ua kom zoo nkauj los ntawm kev pom ntawm cov ximKev loj hlob nyob rau sab hauv sawb. Daim nyias nyias yog immobilized nrog antiCryptococcal monoclonal antibody rau thaj tsam xeem. Thaum lub sijhawm kuaj, hnoos qeevYog tso cai rau kev hnov ​​mob nrog monoclonal los tiv thaiv cryptococcal antibody muaj xim pightralsconjugates, uas tau precoated ntawm conjugate pad ntawm qhov kev xeem. Qhov sib tov cestsiv rau ntawm daim nyias nyias los ntawm kev ua yeeb yam, thiab cuam tshuam nrog reagents nyob rau ntawmmembrane. Yog tias muaj txaus cryptococcal antigens hauv cov qauv, xim ximBand yuav tsim nyob rau ntawm thaj chaw kuaj ntawm daim nyias nyias. Muaj cov band no bandQhia txog qhov txiaj ntsig zoo, thaum nws tsis yog qhov tshwm sim tsis zoo. Kev tshwm simNtawm ib tug xim band ntawm cov cheeb tsam tswj tau ua haujlwm raws li txheej txheem tswj. Qhov no qhia tauuas kom tsim nyog ntim ntawm cov hnoos qeev tau ntxiv thiab membrane wicking muajtshwm sim.

COV KEV CEEV FAJ
Cov khoom siv no yog rau hauv cov vitro kuaj xyuas siv nkaus xwb.
Cov khoom siv no yog rau kev siv kev paub nkaus xwb.
■ Nyeem ua tib zoo nyeem ua ntej ua qhov kev sim.
■ Cov khoom no tsis muaj cov khoom siv tib neeg.
Tsis txhob siv cov khoom siv ua khoom siv tom qab hnub tas sij hawm.
■ Siv tag nrho cov hnoos qeev uas yog kis tau.
Cov txheej txheem ntawm cov txheej txheem biosafety rau kev tuav thiabpov tseg ntawm cov khoom siv sib kis tau sib kis. Thaum cov txheej txheem assayua tiav, muab cov hnoos qeev tom qab autoclaving lawv ntawm 121 ℃ tsawg kawg20 feeb. Hloov pauv, lawv tuaj yeem kho nrog 0.5% Sodium hypochloriterau cov sij hawm ua ntej pov tseg.
Tsis txhob pipette reagent los ntawm qhov ncauj thiab tsis haus luam yeeb lossis noj thaum ua yeeb yamua yeeb yam.
Hnab looj tes rau tag nrho cov txheej txheem.