Siv
Lub zog^®Kev ntsuam xyuas Spcalcitonin yog kev tiv thaiv kab mob sai-Chromatographicassay rau kev kuaj pom semi sevitive uas tau kuaj pom ntawm procalcitonin nyob rau hauv tib neeg mob ntshav lossisntshav. Nws yog siv rau kev kuaj mob thiab tswj kev kho mob ntawm mob hnyav,Kab mob kis tau thiab sepsis.

Lub hauv paus rau phau ntawv
Spralcitonin (PCT) yog cov protein me me uas muaj 116 amino acid seemnrog txoj kev ua molecular ntawm kwv yees li 13 kda uas tau piav qhia thawj zauglos ntawm Moullec li al. Xyoo 1984.PCT yog tsim cov feem ntau hauv cov qog cov qog ua cov qog. Hauv xyoo 1993, tusnce qib ntawm PCT hauv cov neeg mob nrog kev kis tus kab mob kab mobtau tshaj tawm thiab PCT yog tam sim no suav tias yog lub cim tseem ceeb ntawm kev tsis sib haum xeebnrog los ntawm cov kab mob system thiab sepsis. Tus nqi kuaj mob ntawmPCT yog ib qho tseem ceeb vim txoj kev sib cuam tshuam nruab nrab ntawm pct concentration thiabtus mob heev ntawm o. Nws tau qhia tias "mob o" pct tsis yogtsim nyob rau hauv c-cell. Cov hlwb ntawm cov neeg neuroendocrine yog tej yam tseem ceebntawm pct thaum lub caij mob.

Lub ntsiab lus
Lub zog^®Kev ntsuam xyuas sai sai ntes kuaj pom kev tshaj tawm los ntawm kev pomKev txhais cov xim kev txhim kho ntawm txoj hlua sab hauv. ProcalcitoninMonoclonal antibody yog immobilized rau ntawm thaj av sim ntawm daim nyias nyias. ThaumKev kuaj, tus me me reacts nrog monoclonal anti-procalcitonin tiv thaivconjugated rau cov xim sau thiab prepoated mus rau lub conjugate pad ntawm qhov kev xeem.Cov sib tov ces mig los ntawm daim nyias nyias los ntawm capillary ua thiabsib cuam tshuam nrog reagents ntawm daim nyias nyias. Yog tias muaj txaus procalcitonin hauvCov hnoos qeev, cov xim xim yuav ua tiav ntawm thaj chaw kuaj ntawm qhov chaw membrane. Tusmuaj cov kob no band qhia tias muaj txiaj ntsig zoo, thaum nws tsisqhia tias qhov tshwm sim tsis zoo. Cov tsos ntawm cov xim band ntawm kev tswj hwmThaj av pab ua cov txheej txheem kev tswj hwm, qhia tias cov ntim ntim kom zooCov zis tau ntxiv thiab Membrane Wicking tau tshwm sim.Kev tsim kho xim hauv cov cheeb tsam xeem (T) qhia txog qhov txiaj ntsig zoowhereas tus nqi ntawm kwv yees ntawm procalcitonin tuaj yeem txheeb xyuas Semi-Touanitatively los ntawmkev sib piv ntawm cov kab kev xeem rau kev siv cov kab siv siv rau covDaim npav txhais lus. Qhov tsis muaj ntawm kab kob hauv thaj tsam xeem kab (t)qhia cov txiaj ntsig tsis zoo.

COV KEV CEEV FAJ
Cov khoom siv no yog rau hauv cov vitro kuaj xyuas siv nkaus xwb.
■ Nyeem ua tib zoo nyeem ua ntej ua qhov kev sim.
■ Cov khoom no tsis muaj cov khoom siv tib neeg.
Tsis txhob siv cov khoom siv ua khoom siv tom qab hnub tas sij hawm.
■ Siv tag nrho cov hnoos qeev uas yog kis tau.
Cov txheej txheem ntawm cov txheej txheem biosafety rau kev tuav thiabpov tseg ntawm cov khoom siv sib kis tau sib kis. Thaum cov txheej txheem assayua tiav, muab cov hnoos qeev tom qab autoclaving lawv ntawm 121 ℃ tsawg kawg20 feeb. Hloov pauv, lawv tuaj yeem kho nrog 0.5% Sodium hypochloriterau cov sij hawm ua ntej pov tseg.
Tsis txhob pipette reagent los ntawm qhov ncauj thiab tsis haus luam yeeb lossis noj mov whileperforming cia.
Hnab looj tes rau tag nrho cov txheej txheem.