Siv
Lub zog^®Kev ntsuam xyuas Spratcitonin yog kev tiv thaiv sai sai - Chromatographic assay rau semi-senipitative nrhiav tau ntawm procalcitonin nyob rau hauv tib neeg lossis ntshav. Nws yog siv rau kev kuaj mob thiab tswj kev kho mob ntawm kev raug mob hnyav, kab mob thiab sepsis.

Lub hauv paus rau phau ntawv
Protcalcitonin (PCT) yog cov protein me me uas muaj 116 amino acid seem uas muaj kwv yees li 13 kda uas tau hais thawj zaug los ntawm Moullec li al. Xyoo 1984. PCT yog tsim tawm ib txwm nyob hauv C-cell ntawm cov thyroid caj pas. Xyoo 1993, qib siab ntawm PCTs ntawm cov neeg mob kab mob kis tau tshaj tawm thiab PCT yog tam sim no yog lub cim tseem ceeb ntawm kev tsis sib haum nrog kev cuam tshuam nrog cov tshuaj lom neeg thiab sepsis. Qhov kev kuaj mob tus nqi ntawm PCT yog qhov tseem ceeb vim txoj kev sib cuam tshuam nruab nrab ntawm pct concentration thiab tus mob heev ntawm kev o. Nws tau qhia tias "mob o" PCT tsis yog tsim hauv C-cell. Cov hlwb ntawm cov keeb kwm neuroendocrine yog tej yam tseem ceeb ntawm cov pct xwm txheej.

Lub ntsiab lus
Lub zog^®Kev tshawb fawb nrawm nrawm ntes ntes tau tshaj tawm los ntawm kev pom ntawm kev txhim kho xim ntawm sab hauv. Spralcitonin Monoclonal antibodody yog immobilized rau ntawm thaj chaw sim ntawm daim nyias nyias. Thaum kuaj, qhov hnoos qeev uas muaj cov tshuaj tiv thaiv monoclonal los tiv thaiv cov kab mob ntawm cov tshuaj pleev ib ce thiab prepoated mus rau lub conjugate pad ntawm qhov ntsuas. Qhov sib tov ces mig los ntawm daim nyias nyias los ntawm capillary kev ua thiab cuam tshuam nrog reagents ntawm daim nyias nyias. Yog tias muaj cov khoom siv txaus hauv qhov hnoos qeev, cov xim xim yuav tsim nyob rau ntawm thaj chaw kuaj ntawm daim nyias nyias. Lub xub ntiag ntawm cov band no band qhia tau qhov txiaj ntsig zoo, thaum nws tsis muaj qhov tshwm sim tsis zoo. Cov tsos ntawm cov xim muaj xim ntawm cov cheeb tsam tswj tau ua cov txheej txheem kev tswj hwm, qhia tias cov qauv ntim tau zoo tau ntxiv thiab Membrane Wicking tau tshwm sim. Ib qho kev nthuav dav xim hauv cov kab ke xeem (t) qhia txog cov txiaj ntsig zoo los ntawm kev sib piv ntawm cov kab kev siv rau qhov sib piv ntawm daim npav sib txhais. Qhov tsis muaj ntawm kab kob hauv thaj tsam xeem kab (t)
qhia cov txiaj ntsig tsis zoo.

COV KEV CEEV FAJ
Cov khoom siv no yog rau hauv cov vitro kuaj xyuas siv nkaus xwb.
Cov khoom siv no yog rau kev siv kev paub nkaus xwb.
■ Nyeem ua tib zoo nyeem ua ntej ua qhov kev sim.
■ Cov khoom no tsis muaj cov khoom siv tib neeg.
Tsis txhob siv cov khoom siv ua khoom siv tom qab hnub tas sij hawm.
■ Siv tag nrho cov hnoos qeev uas yog kis tau.
Cov txheej txheem ntawm cov txheej txheem ntawm bi biosafety rau kev tuav thiab pov tseg cov khoom muaj peev xwm sib kis. Thaum cov txheej txheem assay ua tiav, muab cov hnoos qeev tom qab autoclaving lawv thaum 121 ℃ tsawg kawg 20 min. Hloov pauv, lawv tuaj yeem kho nrog 0.5% Sodium hypochlorite rau cov teev ua ntej pov tseg.
Tsis txhob pipette reagent los ntawm qhov ncauj thiab tsis haus luam yeeb lossis noj mov whileperforming cia.
Hnab looj tes rau tag nrho cov txheej txheem.