Rotavirus antigen sai xeem
Lub hauv paus rau phau ntawv
Rotavirus yog cov neeg sawv cev feem ntau muaj lub luag haujlwm rau kev mob siab, feem ntau yog cov menyuam yaus. Nws qhov kev tshawb pom nyob rau xyoo 1973 thiab nws cov koom haum nrog cov mob plab-enterite tau sawv cev rau kev kawm txog kev kis tus kab mob. Rotavirus kis tau los ntawm kev txiav txim siab ntawm qhov ncauj nrog kev tsim kom loj hlob ntawm 1-3 hnub. Txawm hais tias cov hnoos qeev uas sau tau hauv qhov thib ob thiab thib tsib ntawm kev mob nkeeg yog qhov zoo tshaj plaws rau cov antigen nrhiav kom tau, rotavirus tseem tuaj yeem pom thaum zawv plab ntxiv. Rotaviral Gastroentitivitis yuav ua rau lub neej no rau cov neeg muaj kev pheej hmoo xws li cov menyuam mos, cov neeg laus thiab immunocompomised cov neeg mob. Hauv huab cua huab cua sov, huab cua huab cua tshwm sim feem ntau nyob rau lub caij ntuj no. Endemics raws li cov kab mob kev cuam tshuam rau qee txhiab tus neeg tau tshaj tawm. Nrog cov menyuam yaus muaj kev txom nyem los ntawm tus kab mob sib koom tes, nce mus txog 50% ntawm cov ntawv txheeb xyuas cov hnoos qeev tau zoo rau kev nplua. Cov kab mob no rov ua dua nyob hauv
Nucleus thiab zoo li yuav yog tus tswv tsev hom-tshwj xeeb ua rau cov txiaj ntsig cytopathic nyhuv (CPE). Vim tias cov kab mob rotavirus yog qhov nyuaj heev rau kev coj noj coj ua, nws yog qhov txawv txav kev sib cais ntawm tus kab mob hauv kev kuaj mob ntawm kev kis mob. Hloov chaw, muaj ntau hom tswv yim tau tsim los txheeb xyuas rotavirus hauv quav.
Lub ntsiab lus
Lub rotavirus nrawm sim ntaus ntawv (cov quav) kuaj pom cov rotavirus los ntawm kev pom kev ntawm cov xim ntawm sab hauv. Anti-roseavirus antibodies yog immobilized rau ntawm thaj chaw sim ntawm daim nyias nyias. Thaum kuaj, hnoos qeev
Rov qab nrog anti-roseavirus antejodies conjugated rau cov xim sau thiab prepoated rau cov qauv ncoo ntawm qhov ntsuas. Qhov sib tov ces mig los ntawm daim nyias nyias los ntawm capillary kev ua thiab cuam tshuam nrog reagents ntawm daim nyias nyias. Yog muaj
Txaus rotavirus hauv cov hnoos qeev, ib band xim xim yuav tsim nyob rau ntawm thaj chaw kuaj ntawm daim nyias nyias. Lub xub ntiag ntawm cov band no band qhia tau qhov txiaj ntsig zoo, thaum nws tsis muaj qhov tshwm sim tsis zoo. Cov tsos ntawm cov kob band ntawm lub
Tswj thaj av pabcuam ua cov txheej txheem kev tswj hwm, qhia tias cov qauv ntim kom zoo tau raug ntxiv thiab Membrane Wicking tau tshwm sim.
Cov Khoom Siv Khoom Siv
| Tus kheej uas tau ntim cov khoom siv | Txhua lub cuab yeej muaj cov sawb nrog cov xim sib dhos thiab reagents reagents ua ntej-coated ntawm cov cheeb tsam sib xws. |
| Zoov Qaum Qwj Tube Nrog Tsis | 0.1 m phosphate buffered saline (PBS) thiab 0.02% sodium azide. |
| Cov Khoom Siv Uas Siv Pov Tseg | Rau kev sau cov kua ua kua |
| Cov ntim ntim | Rau Kev Ua Haujlwm Cov Lus Qhia |
Cov ntaub ntawv yuav tsum tau tab sis tsis muab
| Timer | Rau Kev Siv Sijhawm |
| Centrifuge | Txog kev kho mob ntawm cov zis hauv cov xwm txheej tshwj xeeb |
Ntawv muaj
