Tuam Tshoj Txoj Kev Paub Ntawm Novel Coronavirus Pneumonia's Diagnosis

Qhov twg yog txoj kev zoo tshaj plaws?
-Tests for SARS-CoV-2 Infection Diagnosis

China's Experience At Novel Coronavirus Pneumonia's Diagnosis

Rau cov neeg uas tau lees paub COVID-19, tau tshaj tawm cov tsos mob tshwm sim suav nrog ua npaws, hnoos, myalgia lossis qaug zog.Txawm li cas los xij, cov tsos mob no tsis yog qhov tshwj xeeb ntawm COVID-19 vim tias cov tsos mob no zoo ib yam li lwm cov kab mob sib kis xws li mob khaub thuas.Tam sim no, tus kab mob nucleic acid Real-Time PCR (rt-PCR), CT imaging thiab qee qhov hematology tsis yog cov cuab yeej tseem ceeb rau kev kuaj mob ntawm tus kab mob.Ntau cov khoom siv kuaj sim tau tsim thiab siv hauv kev sim cov neeg mob kuaj rau COVID-19 los ntawm Suav CDC1, US CDC2thiab lwm cov tuam txhab ntiag tug.IgG / IgM tshuaj tiv thaiv kab mob, ib txoj hauv kev kuaj serological, kuj tau ntxiv raws li cov txheej txheem kuaj mob hauv Tuam Tshoj cov kev hloov kho tshiab ntawm kev kuaj mob thiab kev kho mob rau tus kab mob tshiab coronavirus (COVID-19), uas tau tshaj tawm rau hnub tim 3, Lub Peb Hlis.1.Tus kab mob nucleic acid rt-PCR kuaj tseem yog tus qauv ntsuas tam sim no rau kev kuaj mob ntawm COVID-19.

https://www.limingbio.com/sars-cov-2-rt-pcr-product/

StrongStep®Novel Coronavlrus (SARS-COV-2)Multiplex Real-Time PCR Kit (nrhiav peb lub noob)

Txawm li cas los xij cov khoom siv PCR lub sijhawm tiag tiag, nrhiav cov khoom siv caj ces ntawm tus kab mob, piv txwv li hauv qhov ntswg, qhov ncauj, lossis qhov quav qhov quav, raug kev txom nyem los ntawm ntau qhov kev txwv:

1) Cov kev ntsuam xyuas no muaj lub sij hawm tig mus ntev thiab nyuaj rau kev ua haujlwm;lawv feem ntau siv li ntawm 2 mus rau 3 teev los tsim cov txiaj ntsig.

2) Cov kev xeem PCR xav tau cov chaw kuaj mob uas muaj ntawv pov thawj, cov khoom siv kim thiab cov kws tshaj lij kev cob qhia los ua haujlwm.

3) Muaj qee tus lej ntawm qhov tsis zoo rau rt-PCR ntawm COVID-19.Tej zaum nws yuav yog vim muaj tus kab mob SARS-CoV-2 tsawg nyob rau hauv cov kab mob ua pa sab sauv sab sauv (Novel coronavirus feem ntau kis tau rau hauv lub ntsws, xws li pulmonary alveoli ) thiab qhov kev sim tsis tuaj yeem txheeb xyuas cov neeg uas tau kis tus kab mob, zoo, thiab. tshem tus kab mob los ntawm lawv lub cev.

Kev tshawb fawb los ntawm Lirong Zou et al4pom tias muaj cov kab mob ntau dua tau kuaj pom sai sai tom qab cov tsos mob tshwm sim, nrog cov kab mob ntau dua kuaj pom hauv lub qhov ntswg tshaj hauv caj pas thiab cov kab mob nucleic acid los ntawm cov neeg mob kis tus kab mob SARS-CoV-2 zoo ib yam li cov neeg mob khaub thuas.4thiab zoo li txawv ntawm qhov pom hauv cov neeg mob uas muaj SARS-CoV-2.

Yang Pan et al5soj ntsuam cov qauv kuaj (khob caj pas, hnoos qeev, tso zis, thiab quav) los ntawm ob tus neeg mob hauv Beijing thiab pom tias cov kab mob hauv caj pas thiab cov hnoos qeev tau nce siab nyob ib ncig ntawm 5-6 hnub tom qab cov tsos mob tshwm sim, cov hnoos qeev feem ntau pom muaj cov kab mob ntau dua. caj pas swab qauv.Tsis muaj tus kab mob RNA raug kuaj pom hauv cov zis lossis cov quav hauv ob tus neeg mob no.

Kev kuaj PCR tsuas yog muab cov txiaj ntsig zoo thaum tus kab mob tseem nyob.Cov kev ntsuam xyuas tsis tuaj yeem txheeb xyuas cov neeg uas tau kis tus kab mob, rov zoo, thiab tshem tus kab mob ntawm lawv lub cev.Hauv qhov ua tau zoo, tsuas yog kwv yees li 30%-50% tau zoo rau PCR hauv cov neeg mob uas tau kuaj pom tus mob tshiab mob ntsws.Ntau tus neeg mob mob ntsws tshiab mob ntsws tsis tuaj yeem kuaj pom vim qhov kev sim nucleic acid tsis zoo, yog li lawv tsis tuaj yeem tau txais kev kho mob raws sijhawm.Los ntawm thawj zaug mus rau thib rau tsab ntawm cov lus qhia, tso siab rau lub hauv paus ntawm kev kuaj mob ntawm cov txiaj ntsig nucleic acid, uas ua rau muaj teeb meem loj rau cov kws kho mob.Qhov ntxov tshaj plaws "xws li-blower", Dr. Li Wenliang, kws kho mob ntawm lub ntsej muag ntawm Wuhan Central. Tsev kho mob, tuag lawm.Thaum nws lub neej, nws tau kuaj peb lub nucleic acid nyob rau hauv cov ntaub ntawv ntawm kub taub hau thiab hnoos, thiab lub sij hawm kawg nws tau txais PCR zoo.

Tom qab kev sib tham los ntawm cov kws tshaj lij, nws tau txiav txim siab los ua kom cov kev kuaj mob los ua cov kev kuaj mob tshiab.Thaum kuaj tshuaj tiv thaiv kab mob, tseem hu ua serological tests, uas tuaj yeem paub meej tias ib tus neeg puas tau kis tus kab mob txawm tias tom qab lawv lub cev tiv thaiv kab mob tau tshem tawm tus kab mob uas ua rau COVID-19.

China's Experience At Novel Coronavirus Pneumonia's Diagnosis2
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StrongStep® SARS-COV-2 IgG/IgM Antibody Rapid Test

IGG / IGM tshuaj tiv thaiv antibody yuav pab rau kab hauv ntau cov pej xeem uas muaj tus mob asymptomatic uas tsis tuaj yeem txheeb tau yooj yim.Ib khub niam txiv hauv Singapore, tus txiv tau kuaj pom qhov zoo los ntawm PCR, nws tus poj niam qhov kev kuaj PCR tsis zoo, tab sis cov tshuaj tiv thaiv kab mob pom tau tias nws muaj tshuaj tiv thaiv, ib yam li nws tus txiv.

Kev soj ntsuam serological yuav tsum tau ua tib zoo xyuas kom paub tseeb tias lawv hnov ​​​​txog kev ntseeg siab, tab sis tsuas yog rau cov tshuaj tiv thaiv tus kab mob tshiab.Ib qho kev txhawj xeeb yog qhov zoo sib xws ntawm cov kab mob uas ua rau mob ua pa nyuaj thiab COVID-19 tuaj yeem ua rau muaj kev sib kis.Lub IgG-IgM tsim los ntawm Xue Feng wang6tau txiav txim siab tias nws tuaj yeem siv los ua qhov ntsuas qhov ntsuas ntawm qhov chaw zov me nyuam (POCT), vim tias nws tuaj yeem ua tau nyob ze ntawm lub txaj nrog cov ntshav ntiv tes.Cov khoom siv muaj rhiab heev ntawm 88.66% thiab qhov tshwj xeeb ntawm 90.63%.Txawm li cas los xij, tseem muaj cov txiaj ntsig tsis zoo thiab tsis tseeb tsis zoo.

Hauv Tuam Tshoj qhov hloov kho tshiab ntawm kev kuaj mob thiab cov lus qhia kho mob rau tus kab mob tshiab coronavirus (COVID-19)1, cov mob uas tau lees paub yog txhais tau tias yog cov neeg mob xav tias yuav ua tau raws li ib qho ntawm cov hauv qab no:
(1) Cov qauv ua pa, cov ntshav lossis cov quav quav tau kuaj pom zoo rau SARS-CoV-2 nucleic acid siv rt-PCR;
(2) Genetic sequencing ntawm tus kab mob los ntawm cov kab mob ua pa, cov ntshav los yog cov quav cov qauv coj mus kuaj yog homologous heev nrog cov paub SARS-CoV-2;
(3) Cov tshuaj tiv thaiv kab mob coronavirus tshwj xeeb IgM thiab IgG tshuaj tiv thaiv tau zoo;
(4) Cov tshuaj tiv thaiv kab mob coronavirus tshwj xeeb IgG hloov pauv los ntawm qhov tsis zoo mus rau qhov zoo lossis tus kabmob coronavirus tshwj xeeb IgG cov tshuaj tiv thaiv kabmob thaum lub sijhawm rov zoo yog 4 npaug siab dua li lub sijhawm mob hnyav.

Kev kuaj mob thiab kev kho mob ntawm COVID-19

Cov lus qhia

Tshaj tawm

Cov txheej txheem kuaj mob tau lees paub

Version 7th

3 Peb 2020

❶ PCR

❷ NWS

❸ IgM + IgG

Version 6
Version 5th
Version 4th
Version 3rd
Version 2nd
Version 1st

18 Peb. 2020
3 Lub Ob Hlis 2020
27 Lub Ib Hlis 2020
22 Lub Ib Hlis 2020
16 Lub Ib Hlis 2020

❶ PCR

❷ NWS

Kev siv
1. Cov lus qhia rau kev kuaj mob thiab kev kho mob ntawm tus kab mob coronavirus tshiab (sim version 7, National Health Commission of the People's Republic of China, tawm ntawm 3.Mar.2020)
http://www.nhc.gov.cn/yzygj/s7652m/202003/a31191442e29474b98bfed5579d5af95.shtml

2. Kev tshawb fawb siv tsuas yog Real-Time RT-PCR Protocol for Identification of 2019-nCoV
https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html

3. Singapore thov thawj zaug siv tshuaj tiv thaiv kab mob txhawm rau taug qab tus kabmob coronavirus
Tau qhov twg los: https://www.sciencemag.org/news/2020/02/singapore-claims-first-use-antibody-test-track-coronavirus-infections

4.SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients February 19,2020 DOI: 10.1056/NEJMc2001737

5.Viralloads of SARS-CoV-2 in clinical samples Lancet Infect Dis 2020 Published Online February 24, 2020 (https://doi.org/10.1016/S1473-3099(20)30113-4)

6. Developmentand Clinical Application of A Rapid IgM-IgG Combined Antibody Test for SARS-CoV-2
Kev kuaj kab mob XueFeng Wang ORCID iD: 0000-0001-8854-275X


Post lub sij hawm: Mar-17-2020